Mullen, K.M., Schneider, S.A., & Normark, B.B. 2016 New Single-Copy Nuclear Genes for use in Scale Insect Systematics. . Redia XCIX, 2016: 207-214

Keywords:

DNA sequencing

genetics

Notes: We used transcriptome data for two diaspidids, Acutaspis umbonifera (Newstead) and Chrysomphalus aonidum (Linnaeus), together with a published aphid genome, to design novel PCR primer sets for scale insects. Our primers amplify fragments of eight single-copy genes. Here we report the results of tests of amplification success and phylogenetic utility of these primer sets across the Diaspididae and nine other families of Coccomorpha.