Huang, P.-Y., Lin, L.-H., Fang, Z.-P., Lin, L.-L., & Liao, F.-R. 2017 Identification of Dysmicoccus neobrevipes Beardsley (Hemiptera: Pseudococcus) using 28S rDNA species-specific primers. Chinese Journal of Applied Entomology 54(4): 646-651.
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Notes: Dysmicoccus neobrevipes Beardsley, a new invasive species in China, is a worldwide pest that poses a serious threat to agriculture and forestry. Morphological identification of this species is limited by its close similarity to other mealybug species. To address this problem a new method for the rapid identification of D. neobrevipes was developed and a pair of 28S rDNA species-specific primers (D.neo-28SF/R) were designed. The 28S rDNA genes of D. neobrevipes and six other mealybug species were amplified and sequenced. The diagnostic PCR assay established here provides a quick, simple and reliable molecular technique for identification.
